Mouse lymph nodes contain resident and migratory DNGR-1⁺ CD8α⁺-like DCs. (A) Flow cytometric analysis of cutaneous draining lymph node cell suspensions from B6 mouse. Live cells were analyzed for the expression of CD11c versus MHCII (top right panel). Two populations were defined (gate I, CD11c^high MHCII^inter tissue-resident DCs; gate II, CD11c^{inter to high} MHCII^high migratory DCs) and analyzed for the expression of, respectively, CD8 versus CD11b (for gate I), or CD103 versus CD11b (for gate II, top left panels). Four populations were defined (gate A, CD8⁺, CD11b⁻; gate B, CD8⁻, CD11b⁺; gate C, CD103⁺, CD11b⁻; and gate D, CD103⁻, CD11b⁺) and analyzed for the expression of DNGR-1 (red) or for an isotype control antibody (blue). (B) CD11c^{inter to high} MHCII^high migratory DCs were analyzed for the expression of CD207 versus DNGR-1 (top left panel). Two populations were defined (blue gate, CD207⁺, DNGR-1⁻; and red gate, CD207⁺, DNGR-1⁺) and analyzed for the expression of CD103 versus CD11b. Migratory DCs were analyzed also for the expression of CD103 versus DNGR-1 (bottom left panel). Two populations were defined (blue gate, CD103⁻, DNGR-1⁻; and red gate, CD103⁺, DNGR-1⁺) and analyzed for the expression of CD207 and CD11b. Numbers indicate percentage of cells in each of the indicated gates. Arrows indicate gating strategy. (A-B) Data are representative of 2 independent experiments.