DNGR-1⁺ CD103⁺ CD11b⁻ DCs are found in mouse nonlymphoid tissues. (A) CD11c⁺ MHCII⁺ CD45⁺ live kidney cells from Batf3-sufficient mice (B6; top left panel) or Batf3^−/− mice (bottom left panel) were analyzed as in Figure 1 for the expression of CD103 versus CD11b. For the Batf3^+/+ mice, 2 populations were defined: gate A, CD103⁺, CD11b⁻; and gate B, CD103⁻, CD11b⁺. For the Batf3^−/− mice, only 1 DC gate was defined encompassing gates A and B. Cells within these gates were analyzed for staining with anti–DNGR-1 (black) or an isotype-matched control antibody (grey). (B) CD11c⁺ MHCII⁺ CD45⁺ auto^low live lung cells were analyzed as described for the kidney. Numbers indicate percentage of cells in each of the indicated gates. Arrows indicate gating strategy. (A-B) Data are representative of 2 independent experiments.