In vitro generated DNGR-1⁺ BDCA3⁺ CBDCs are Batf3-dependent. CBDCs were generated and infected with different sh-RNA LVs as described in “LV production.” (A) Lin⁻ (CD3/14/16/19/20/56) live cells were analyzed for the expression of HLA-DR and GFP. HLR-DR⁺ GFP⁺ cells were analyzed for the expression DNGR-1 versus CD11b, BDCA3 versus DNGR-1, and BDCA3 versus CD11b. (B) Frequency of DNGR-1⁺ BDCA3⁺ GFP⁺ (left panel) and of CD11b⁺ GFP⁺ cells (right panel). (A-B) Data are representative of multiple CBDC cultures with 2 independent pools of CB-derived HSCs/HPCs.