Ex vivo generated DNGR-1⁺ BDCA3⁺ from humanized mice are Batf3-dependent. (A) BMDCs were generated as described in “In vitro–differentiated DCs” from bone marrow of humanized mice and analyzed as in Figure 5. (B) Frequency of DNGR-1⁺ BDCA3⁺ GFP⁺ (left panel) and of CD11b⁺ GFP⁺ cells (right panel). Numbers indicate percentage of cells in each of the indicated gates. (A) One representative experiment of 2 is shown, with 4 mice for each sh-RNA. (B) Data are mean ± SEM of 2 independent experiments. *P < .05 (Mann-Whitney test). **P < .01 (Mann-Whitney test). ***P < .001 (Mann-Whitney test). sh-CTRL indicates control.