Batf3 silencing is not sufficient to impair DNGR-1⁺ BDCA3⁺ DC development in humanized mice. (A) CD11c⁺ HLA-DR⁺ live cells (same as in Figure 3) were analyzed for the expression of BDCA3 versus CD11b (top panel) among the GFP⁺ cells. Two populations were identified: gate I, BDCA3⁺, CD11b⁻; and gate II, BDCA3⁻, CD11b⁺. Numbers indicate percentage of cells in each of the indicated gates. (B) Frequency of BDCA3⁺ GFP⁺ (top panel) and of CD11b⁺ GFP⁺ cells (bottom panel) is shown. (C) Normalized expression of Batf3 within Lin⁻ (CD3/14/16/19/20/56) HLA-DR⁺ GFP⁺ live cells, purified from the spleen of the humanized mice. (A,C) Data are representative of 2 independent experiments, with 4 mice for each sh-RNA. (B) Data are mean ± SEM of 2 independent experiments. *P < .05 (Mann-Whitney test). sh-CTRL indicates control; Sp, spleen; Lg, lung; and K, kidney.