LMO2 effects on cell proliferation, and centrosome number. OCILy1 cells transduced with doxycycline inducible LMO2 lentivirus were left un-induced or were exposed to the indicated concentrations of doxycycline for specified time periods. (A) Proliferation analyzed by an MTS assay. Results are shown as the means ± SD and are representative of 3 independent experiments. Western blot confirms dose-dependent induction of LMO2 protein at 24 hours after addition of doxycycline. (B) The cells were spun down on slides, fixed and permeabilized as described in “Centrosome quantification,” and stained with γ-tubulin antibody and DAPI. Centrosome number was counted in at least 100 cells per experiment and percentage of cells with 2 or less, and 3 or more centrosomes per cell is shown. The presented results represent the average from 3 independent experiments (*P < .001 compared with control). (C) Examples of immunofluorescence centrosome staining visualized with a Zeiss Apotome microscope with 63×/1.4 NA plan apochromat objective lens, using software AxioVision LE. For details see “Centrosome quantification.”