LMO2 interacts with LEF1, ELK1, and NFATc1 proteins. (A) Protein expression in nuclear extracts of DLBCL cell lines was assayed by immunoblotting with specific antibodies. Immunoblotting for Histone 3 served as a loading control. (B) Nuclear extracts were prepared from SU-DHL-6 and OCILy19 cell lines and used for immunoprecipitation with LMO2 antibody and immunoblotting with antibodies to indicated proteins. Results in panels A and B are representative of 2 independent experiments. (C) NFAT-driven luciferase reporter construct (10 μg), and either the NFATc1 (5 μg), LMO2 (5 μg), or both plasmids were coexpressed in the DOHH2 cells. Luciferase activity was determined 48 hours after transfection. The values are relative luciferase activities, with the average value obtained for the same amount of reporter and pcDNA3.1 control plasmid set at 1. Values are means + SE of 3 independent experiments, each performed in 3 replicate samples. (D) LEF1-driven luciferase reporter construct (3 μg), and either the LEF1 (10 μg), LMO2 (5 μg), or both plasmids were coexpressed in the Raji cells. Luciferase activity was determined 48 hours after transfection. The values are relative luciferase activities, with the average value obtained for the reporter using same amount of pcDNA3.1 control plasmid set at 1. Values are means plus standard error (error bars) of 3 independent experiments, each performed in 3 replicate samples.