Levels of TF expression in 4 human pancreatic cell lines. TF mRNA (A) was measured using RT-PCR and TF protein (B) was measuring by Western blot. GAPDH was used as the loading control. TF protein expression on the cells was determined by flow cytometry using a PE-conjugated HTF-1 Ab (C). Levels of TF protein (D) was measured by ELISA and TF activity (E) was measured by a 1-stage clotting assay (n = 3).