Analysis of circulating levels of TF protein, MP TF activity, PS-positive MPs, and activation of coagulation in mice with different human pancreatic tumors. Four pancreatic cancer cell lines (MIAPaCa-2, PANC-1, HPAF-II, and HPAC) were injected into the pancreas of nude mice and tumors were grown for 8 weeks (n = 4-11 mice per group). Blood was collected from the IVC and plasma was prepared. TF activity of the tumor lysate (A) and levels of plasma human TF protein (B), human TF activity in MPs (C), PS-positive MPs (D), TAT (E), and D-dimer (F) were measured and are shown as dot plots with means ± SEM. Differences between groups were analyzed using 1-way ANOVA with the Dunn multiple comparison test. *P < .05 control versus HPAF-II (C-F) or HPAC (E-F); #P < .05 HPAF-II versus HPAC (E-F).