Figure 3
Figure 3. Inhibition of PF4 tetramerization. (A) SDS-PAGE of PF4 cross-linked with BS3 (or uncross-linked controls) with or without antagonists (lane 1, WT-PF4; lane 2, WT-PF4 cross-linked; lane 3, PF4K50E; lane 4, PF4K50E cross-linked; lanes 5-9, WT-PF4 cross-linked in the presence of antagonist: lane 5, 250μM PF4A01; lane 6, 500μM PF4A17; lane 7, 250μM PF4A31-01; lane 8, 250μM PF4A31-04; lane 9, 500μM PF4A35; lane 10, molecular mass markers). Arrows denote PF4 monomers (M), dimers (D), and tetramers (T). (B) Dose-response inhibition of PF4 tetramer formation by antagonists. Data are the mean ± SEM of at least 3 independent experiments. Curves represent fit of data to the indirect Hill equation.

Inhibition of PF4 tetramerization. (A) SDS-PAGE of PF4 cross-linked with BS3 (or uncross-linked controls) with or without antagonists (lane 1, WT-PF4; lane 2, WT-PF4 cross-linked; lane 3, PF4K50E; lane 4, PF4K50E cross-linked; lanes 5-9, WT-PF4 cross-linked in the presence of antagonist: lane 5, 250μM PF4A01; lane 6, 500μM PF4A17; lane 7, 250μM PF4A31-01; lane 8, 250μM PF4A31-04; lane 9, 500μM PF4A35; lane 10, molecular mass markers). Arrows denote PF4 monomers (M), dimers (D), and tetramers (T). (B) Dose-response inhibition of PF4 tetramer formation by antagonists. Data are the mean ± SEM of at least 3 independent experiments. Curves represent fit of data to the indirect Hill equation.

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