MINK1^−/− mice display impaired thrombus formation in vitro and in vivo. (A) Representative images and time courses of thrombus formation induced by FeCl₃ injury to mesenteric arterioles in WT (top row) and MINK1^−/− mice (bottom row). a, arteriole; v, venule. (B) Dot plot showing occlusion times for arterioles as a result of FeCl₃-induced thrombosis in WT (n = 9) and MINK1^−/− mice (n = 10). Means are indicated by horizontal lines. **P < .01, 2-tailed Mann-Whitney test. (C) Photomicrographs showing the progression of adhesion of platelets from WT and MINK1^−/− mice on collagen. Whole blood from WT and MINK1^−/− mice, collected in heparin (7.5 U/mL), was fluorescently labeled by incubation with mepacrine (100 μM) for 30 minutes, and then perfused through fibrillar collagen-coated bioflux plates at a shear rate of 40 dynes/cm² for 5 minutes. Original magnification, ×20. (D) Dot plot showing area coverage of platelets from WT and MINK1^−/− mice (n = 3 for each group; *P < .05, Student t test).