MINK1 regulates platelet spreading, but not clot retraction. (A) Spreading of WT and MINK1^−/− platelets on immobilized fibrinogen in the presence or absence of apyrase (1 U/mL) or ADP (1 μM). Images are representative of 3 independent experiments with similar results. Original magnification, ×100. (B) Quantification of the areas of spread (μm²) WT and MINK1^−/− platelets (mean ± SEM; ***P < .001, Student t test). (C) Platelets from WT or MINK1^−/− mice were resuspended with human platelet-poor plasma at a concentration of 4 × 10⁸/mL, and recombined plasma was stimulated to coagulate with thrombin (0.4 U/mL), then photographed at different time points. Experiments were repeated at least 3 times.