Reduced colony formation of myeloid progenitors on A1 knockdown. (A) A total of 2 × 10⁴ cells derived from bone marrow from WT, TREA1, or DT mice, sorted into eGFP⁻, eGFP⁺, and eGFP⁺⁺ fractions, or (B) WT and VVA1.2 mice were plated in Methocult medium in the presence of G-CSF, IL-6, and IL-3. (C-D) Alternatively, cytokines were added after a 24-hour delay. Colonies were counted blinded after 7 days of incubation. Data are mean ± SE of 3 independent experiments performed in duplicates. (E) Bone marrow cells were isolated from single-transgenic TREA1 or DT mice that had been treated for 32 days with doxycycline in the drinking water. Cells were isolated and seeded into cytokine-complemented Methocult medium in the absence or presence of doxycycline. (F) Alternatively, cytokines were added after 24-hour delay. Colonies were counted in duplicate cultures blinded after 7 days of incubation. Data are mean ± SD of 2 or 3 mice per genotype. ANOVA followed by Fisher PLSD posthoc test: *P ≤ .05, compared with WT or single-transgenic controls; and **P ≤ .01, compared with WT or single-transgenic controls.