Expansion of CD56⁺CD3⁻ NK cells with dasatinib from cord blood cells. (A) The absolute number of CD56⁺CD3⁻ NK cells had significantly increased in the culture with dasatinib compared with those in the culture with cytokines only. (B) The proportion of CD56⁺CD3⁻, CD56⁺NKG2D⁺, and CD56⁺granzyme⁺ cells significantly increased after culture with dasatinib compared with culture without dasatinib. (C) After 24 hours, Eomes expression was significantly increased in cord blood cells cultured with dasatinib compared with that in cells cultured with cytokines only. T-bet expression was increased after 24 hours culture compared with that before culture, but there was no significant difference between expression level in culture with dasatinib and that without dasatinib (bars indicate means ± SDs, n = 6; *P < .05 and **P < .01).