CCR9⁻ pDC-like precursors acquire a cDC-like phenotype 48 hours after transfer. Analysis of BST2 and CD11c expression in Violet trace⁺ cells in spleen, lymph nodes, and Peyer patches 48 hours after transfer of CCR9⁻ pDC-like precursors by flow cytometry (dot blots on the left, numbers indicate percentages of BST2^high and BST2^low cells). Analysis of Siglec-H, CCR9, MHCII, and CD11b expression (right panel, histograms) in the BST2^high and BST2^low fractions of Violet trace⁺CD11c⁺ cells 48 hours after transfer of CCR9⁻ pDC-like precursors in spleen, lymph nodes, and Peyer patches (open histograms). Filled histograms indicate unstained control. Numbers in histograms indicate mean fluorescence intensity (MFI). Results of 1 representative of 3 experiments are shown.