Reduced integrin activation and α granule release in RhoA^−/− platelets on G₁₃ and G_q stimulation. Flow cytometric analysis of integrin αIIbβ3 activation (binding of JON/A-PE; A) and degranulation-dependent P-selectin exposure (B) in response to the indicated agonists in wt and RhoA^−/− platelets. Data are mean fluorescence intensities (MFI) ± SD of 4 mice per group and representative of 4 individual experiments (*P < .05; **P < .01; ***P < .001). CVX indicates convulxin; and RC, rhodocytin.