Transplantation of IDO1−/− donor pDCs resulted in altered proportions of donor T-reg and inflammatory donor T cells. B10.BR mice were lethally irradiated and transplanted with 3 to 5 × 103 FACS-sorted HSCs (CD45.2+) and 3 × 105 T cells from B6 (CD45.1+) donors with 5 × 104 FACS-purified pDCs either from WT or IDO1−/− B6 donors. The experiment was performed 4 times with 3 to 5 recipients per group, for a total n = 14 for the HSCs + T group, n = 13 for the group with added WT pDCs, and n = 16 for recipients of IDO1−/− pDCs. Recipient spleens were harvested on day 10 after transplant for quantification of donor T-cell populations and cytokine expression analysis. (A-E) Averages and SEM for each group. (A) Total numbers of CD4 and CD8 donor T cells in recipient spleens. (B) Total numbers of CD8 donor T cells expressing Granzyme and Perforin, and total numbers of donor CD4+CD25+FoxP3+ T-regs (Tregs). (C-E) Percentage of donor CD4 and CD8 T cells expressing IFN-γ (C), TNF-α (D), and IL-17 (E). (F) Ratio comparing the average numbers of IL-17+ CD4 T cells in recipient spleens to the average numbers of CD4+CD25+FoxP3+ T-regs.
