Interaction of platelets with increasing concentrations of wtD′A3 T1255-, Clus1, and DC-D′A3. Flow slides were coated with wtD′A3 and T1255A, Clus1 and DC-D′A3 at concentrations from 2 to 30 μg/mL, and labeled platelets were perfused at 1500 s−1 for 4 minutes. Videos were recorded in real time at a rate of 20 frames per second and 35 frames were superimposed using the high-intensity Z-projection function in ImageJ to visualize the nature of interaction between platelet and VWF. Representative images are shown. Examples of transient tethering events are indicated with white arrowheads, and platelet rolling is indicated with double black arrows. Platelets flowing near the surface, but not interacting, are visible as a white smears (white long arrow).
