Figure 3
Figure 3. BMPRIA expression during NK cell development. (A) Thymic CD34+CD1a− BMPRIA− precursor cells were cultured with SCF and IL-15 for different days and then analyzed by flow cytometry for the expression of CD34, CD161, BMPRIA, CD56, CD94, CD117, and NKp46. Representative phenotypic analyses of total cells harvested at different days of culture are shown. Percentages of positive cells are indicated in dot plots and histograms. (B) Quantitative RT-PCR analysis of the expression of BMPRIA and genes relevant for NK cell development was performed on cells derived from thymic precursors cultured with SCF and IL-15 for different days. Gene expression levels were normalized to GNB2L-1 mRNA content. The data are representative of at least 3 separate experiments.

BMPRIA expression during NK cell development. (A) Thymic CD34+CD1a− BMPRIA− precursor cells were cultured with SCF and IL-15 for different days and then analyzed by flow cytometry for the expression of CD34, CD161, BMPRIA, CD56, CD94, CD117, and NKp46. Representative phenotypic analyses of total cells harvested at different days of culture are shown. Percentages of positive cells are indicated in dot plots and histograms. (B) Quantitative RT-PCR analysis of the expression of BMPRIA and genes relevant for NK cell development was performed on cells derived from thymic precursors cultured with SCF and IL-15 for different days. Gene expression levels were normalized to GNB2L-1 mRNA content. The data are representative of at least 3 separate experiments.

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