Construction and evaluation of human FVII AAV vectors. (A) Schematic of the human FVII constructs. In scAAV-LP1-FVII-wt, the coding region of the human FVII cDNA without the 3′ and 5′ UTRs was inserted into our scAAV backbone downstream of the LP1 and followed by the SV40 virus large T Ag poly-A signal. scAAV-LP1-FVII-coop is identical to scAAV-LP1-FVII-wt except that the wild-type human FVII cDNA has been replaced with a codon-optimized cognate. (B) Genomic configuration of the scAAV-LP1-FVII-wt and scAAV-LP1-FVII-coop provirus is shown under native (left) or alkaline (right) conditions.