Induction of leukemia in CREB transgenic mice by RIM. (A) Kaplan-Meier survival curve of WT and TG mice inoculated with MOL4070LTR retrovirus. Survival curve analysis showed significant difference between WT and TG MOL4070LTR-inoculated mice (P < .001 by log-rank test). (B) Characteristics of leukemia in mice infected with MOL4070LTR retrovirus. Transgenic mice have a 5-month decrease in median latency for disease compared with WT mice inoculated with the MOL4070LTR retrovirus. Both WT- (n = 21) and TG (n = 34)–infected mice that developed leukemia had greater hepatosplenomegaly and increased WBC compared with uninfected WT and TG mice (P < .002). (C) Enlargement of hematologic organs in leukemic mice infected with the MOL4070LTR retrovirus. Leukemic mice typically exhibit enlarged spleens and liver. (Left) Arrows point to enlarged liver (L) and spleen (S) of mouse with AML. (Right) Spleen from WT age-matched control (left side) and CREB TG mouse with AML (right side). (D) Wright-Giemsa–stained slide of peripheral blood and bone marrow from CREB TG mice with AML. This is a representative mouse with myeloid leukemia.