Sox4 overexpression enhances survival and cell-cycle progression of mouse bone marrow cells in vitro. (A) Flow cytometry analysis of retrovirally transduced and sorted WT and TG bone marrow cells grown in the absence of cytokines and limited serum overnight. Sox4 transduction decreases apoptosis and enhances survival of bone marrow cells. (B) Graphical representation of panel A (ANOVA test, ***P < .0001). Tukey test shows a significant difference (P ≤ .05) between all the groups in live and annexin⁺7AAD⁺ double-positive populations. These data represent 3 separate experiments. (C) Cell-cycle analysis by BrdU incorporation. (Top) Percentage of cells in S phase. (Bottom right) Percentage of cells in G₂/M. (Bottom left) Percentage of cells in G₀ phase. (D) Graphical representation of 2 independent BrdU cell-cycle experiments (ANOVA test, ***P < .01). Tukey after ANOVA, P values are represented above the data bars (*P < .05, **P < .01). These results are representative of 2 independent experiments.