Real-time PCR analysis of sox4 expression in mouse bone marrow transductants and adult hematopoiesis. Real-time PCR analysis of creb (A), sox4 (B), cyclin d1 (C), bcl2 (D), and c-fos (E) genes in mouse bone marrow cells, transduced with pMIG-R1 vector or the pMIGR1-sox4 construct. WV = WT + vector, WS = WT + sox4, TV = CREB transgenic + vector, TS = CREB transgenic + sox4 (Tukey *P < .05, **P < .01 ***P < .001). (F) QRT-PCR expression analysis of SOX4 in LSK HSCs, LSK CD150 HSCs, CMPs, GMPs, megakaryocyte erythroid progenitors (MEPs), and differentiated cells. Relative fold is in comparison to Gr-1Mac-1 double-positive myeloid cells. These data are representative of 3 experiments. (G) Sox4 expression in LT-HSCs, ST-HSCs, and MPPs and LSK HSCs and LSK CD150 HSCs for comparison. Relative fold shown is in relation to LSKCD150 HSC. Tukey after ANOVA, P values are represented above the data bars (*P < .05, **P < .01, ***P < .001). These results are from 2 independent experiments. (H) Sox4 binds to the CREB promoter. K562 cells (left) and KG1 cells (right) were cross-linked, harvested, and sonicated, and then chromatin was immunoprecipitated with either anti-Sox4 antibody or IgG control antibody. PCR was performed with primers against CREB promoter region. Input DNA purified from precleared chromatin was used as PCR positive control.