LMD isolatesPCs undergoing tumor progression in inflammatory peritoneal granulomas. (A-C) Low-power images (originally using 10× objective) of peritoneal OGs containing PCs analyzed at early (day 7), middle (day 39), and late (day 107) times after IP injection of 0.5 mL of pristane oil. Paraffin-embedded formalin-fixed tissues were sectioned and stained with Ab against mouse Ig κ light chains to identify PCs (red), and then counterstained with hematoxylin to identify stromal cells (blue). (D-K) Microscopic images of MGP-stained frozen sections of pristane granulomas at early (day 17), middle (days 33 and 46), and late (day 104) times after IP pristane before (D-G) and after (H-K) collection of PCs using LMD. The contours of the tissue fragments targeted by the laser beam are indicated by red lines in panels D through G. The actual tissue fragments collected by LMD came from the holes seen in panels H through K. Microscopic slides were read using an Olympus BX-51 Light Microscope equipped with UPLSAPO objectives (Olympus) of the following magnifications and numerical apertures: 10×, 0.4 (panels A-C); 20×, 0.75 (panels D,H,F,J); 40×, 0.95 (panels E,I,G,K). The imaging medium was air. The light temperature of the microscope bulb varied between 3000 and 3400 K. Images were acquired with the help of a DP2 digital camera (Olympus) and DP2-BSW imaging software (Olympus), saved as TIF (tagged image file) data files, and enhanced—with respect to brightness, contrast, and color balance—using the Adobe Photoshop CS2 Version 9.0.2 software (Adobe Systems Inc).