Paneth cell injury in GVHD. Lethally irradiated B6D2F1 mice were transplanted with 5 × 106 TCD BM cells without (control group, n = 6) or with 2 × 106 T cells (GVHD group, n = 12) from MHC-mismatched B6 donors on day 0. (A-B) Survival (A) and clinical GVHD scores (B) means ± SE are shown. Data from 2 independent experiments were combined. (C-F) Small intestines were isolated from mice 7 days after BMT. (C) Top panels: histology of the small intestine stained with H&E. Bottom panels: Lysozyme staining (brown). Magnification:100×. Bars, 100 μm. (D) Confocal cross-sectioning of the isolated small intestinal crypt. Lysozyme (green) is expressed by Paneth cells. Tetramethyl DAPI (blue) stains the nucleus and phalloidin (red) stains F-actin. Magnification: 1000×. Bars, 10 μm. (E) Pathology scores of the small intestine (mean ± SE, n = 3-6 / group). (F) Quantification of Paneth cells per crypt (mean ± SE, n = 3-6 / group; *P < .05).
