Figure 2
Decreased expression of Paneth cell–derived α-defensins in GVHD. Lethally irradiated B6D2F1 mice were transplanted with 5 × 106 TCD BM without (control group) or with (GVHD group) 2 × 106 T cells from B6 donors. Small intestines were isolated from mice 7 days after BMT. (A) Immunohistochemical staining for defensin α1 (brown). Magnification: 100×. Bars, 100 μm. (B) RNA was extracted from samples and quantitative real-time PCR analysis for enteric defensins including Defa1, Defa4, Defa5, Defa21,22, and Defcr-rs1 was performed (n = 6 / group). Data are representative of 2 similar experiments and are shown as mean ± SE (*P < .05).
