Delayed impairment of the intestinal ecology after MHC-matched BMT. B6 mice were transplanted with 5 × 106 TCD BM without (control group) or with (GVHD group) 2 × 106 T cells from C3H.Sw donors after 12 Gy TBI (n = 6/group). (A-B) Survival (A) and clinical GVHD scores (B, mean ± SE) in control group and GVHD group. Data are representative of 3 similar experiments. (C-F) Fecal pellets were collected once per week after BMT and intestinal microflora was characterized by RFLP analysis of 16S rRNA genes constructed from each sample of fecal pellets and digested with HhaI. Time course changes in flora diversity determined by Simpson index (C), Shannon index (D), and numbers of OTUs (E). (F) Time course changes in the proportion of E coli. Data are representative of 3 similar experiments and are shown as mean ± SE (*P < .05).
