Survival requires integration of VEGF and integrin signaling through Shc. (A) HUVECs were seeded on FN- or CL-coated dishes, and then serum starved for 24 hours with or without 100 ng/mL of VEGF to induce apoptosis. Lysates were immunoblotted for cleaved caspase 3 and GAPDH as a loading control. Survival was quantified by comparing the amount of cleaved caspase 3 present in lysate. Values shown are means ± SEM (n = 3 independent experiments).