FcγRIIa ligation enhances cytokine transcription and activates caspase-1. (A) Relative mRNA expression was determined in DCs that were not stimulated, stimulated with plate-bound IgG, LPS, PGN, or a combination of LPS or PGN with plate-bound IgG. Lysates were made at the indicated time points, after which mRNA expression of the indicated genes was determined by real-time PCR. Expression is normalized to GAPDH and set at 1 for unstimulated cells at 0 hours. Data shown are from 1 representative experiment of 5 experiments using different donors. (B) Caspase-1 activation was determined in DCs that were unstimulated, stimulated with plate-bound-IgG, LPS, PGN, or a combination of LPS or PGN with plate-bound IgG. After stimulation at indicated time points, cells were washed and incubated with a fluorescent caspase-1 inhibitor for 1 hour, after which cells were washed and fluorescence was measured using flow cytometry. Data shown are from 1 representative experiment of 3 experiments using different donors.