Figure 6
Figure 6. KR acts intracellularly and requires adenosine kinase activity to inhibit leukemia cell growth. (A) NBTG and (B) ABT-702 prevented the ability of KR to inhibit TEX cell growth. (C) Inhibitors of nucleobase uptake (NBTI-red squares; NBTG-blue triangles; DiPy-green inverted triangles), adenosine kinase (ABT-702-orange diamonds; 5-ITu-pink open circles; A-134974-brown open squares), adenylate kinase (Ap5A-open yellow triangles), adenylate cyclase (SQ22536-open gray diamonds), and an adenylate cyclase activator (NKH477-open blue inverted triangles) were tested at 500nM in the presence of KR. (D) TEX cells were cultured in the presence of Compound C (black circles), metformin (red squares), AICAR (blue triangles), rapamycin (green inverted triangles), compound 401 (orange diamonds), or PI-103 (pink open squares). Growth inhibition was measured using Alamar Blue. (E) Synergism between kinetin riboside and 10μM of Compound C (black circles), metformin (red squares), AICAR (blue triangles), rapamycin (green inverted triangles), compound 401 (orange diamonds), PI-103 (pink open squares), or DMSO vehicle (brown open squares) was tested using Alamar Blue. (F) Rapamycin was tested on 11 primary AML samples using Alamar Blue.

KR acts intracellularly and requires adenosine kinase activity to inhibit leukemia cell growth. (A) NBTG and (B) ABT-702 prevented the ability of KR to inhibit TEX cell growth. (C) Inhibitors of nucleobase uptake (NBTI-red squares; NBTG-blue triangles; DiPy-green inverted triangles), adenosine kinase (ABT-702-orange diamonds; 5-ITu-pink open circles; A-134974-brown open squares), adenylate kinase (Ap5A-open yellow triangles), adenylate cyclase (SQ22536-open gray diamonds), and an adenylate cyclase activator (NKH477-open blue inverted triangles) were tested at 500nM in the presence of KR. (D) TEX cells were cultured in the presence of Compound C (black circles), metformin (red squares), AICAR (blue triangles), rapamycin (green inverted triangles), compound 401 (orange diamonds), or PI-103 (pink open squares). Growth inhibition was measured using Alamar Blue. (E) Synergism between kinetin riboside and 10μM of Compound C (black circles), metformin (red squares), AICAR (blue triangles), rapamycin (green inverted triangles), compound 401 (orange diamonds), PI-103 (pink open squares), or DMSO vehicle (brown open squares) was tested using Alamar Blue. (F) Rapamycin was tested on 11 primary AML samples using Alamar Blue.

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