Cell populations present in IK^L/L Rag^−/− mice. (A-B) Total cells from the BM, spleen, and lymph nodes (A) or 40% Percoll gradient-enriched cells from the lung and liver (B) of C57BL/6, IK^+/+ Rag^−/−, or IK^L/L Rag^−/− mice were stained and analyzed by flow cytometry. pDCs were gated on CD317⁺ CD11c^low B220⁺. Numbers indicate the percentage of cells among total live cells from the indicated organ. Data shown are representative of at least 3 experiments. (C-D) Spleen (C) and lymph node (D) cells from the indicated mouse strains were stained and analyzed by flow cytometry. Total cell numbers (left column) and percentages (right column) of monocytes/macrophages (Mos/Mfs; F4/80⁺ CD11c⁻ CD11b^high Ly6C⁻ GR1⁻), PMBs/PMEs (SSC^high F4/80⁺ CD11c⁻ CD11b^int Ly6C⁺ GR1^low), and PMNs (F4/80^low CD11c⁻ CD11b⁺ Ly6C⁺ GR1^high) among total live cells are shown. Each dot represents results obtained from individual mice, and bars represent the mean. Data shown are from 3 experiments. *P < .05. ***P < .001.