The in vivo production of cytokines and chemokines after TLR9 stimulation is impaired in the absence of pDCs. C57BL/6, IK^+/+ Rag ^−/−, and IK^L/L Rag^−/− mice were injected with CpG-Dotap (A) or PIC (B), and sera were collected at different time points after injection. Levels of IFN-α, IFN-β, IL-12p40, IL-6, TNF-α, G-CSF, CCL2, CCL3, CCL4, CCL5, CXCL9, and CXCL10 were determined using xMAP. Data shown are from 2 experiments.