Defect in intraperitoneal cell recruitment in the absence of pDCs after intraperitoneal injection of CpG-Dotap. C57BL/6, IK^+/+ Rag^−/−, and IK^L/L Rag^−/− mice were injected intraperitoneally with either PBS or 200 μg of CpG-Dotap. After 1, 2, or 3 days, the total number of CD45⁺ intraperitoneal cells (A) and the number of cells in each indicated population at day 2 (B) were determined (Mos/Mfs indicates monocytes/macrophages). Cell subsets were characterized as shown in supplemental Figure 5. Each dot represents results obtained from individual mice, and bars represent the mean. Data represent the cumulative results of 3 experiments. ns indicates not significant (P > .05). *P < .05. **P < .01. ***P < .001.