Inhibition of PGE₂ biosynthesis reduces DC and DC-committed progenitor cells in mouse BM and spleen. (A left) Representative flow plots show gating strategy to determine DCs in BM (top) and spleen (bottom) of mice treated with indomethacin. (Right) DC frequency of nucleated cells and total DCs per femur (top) and spleen (bottom; mean ± SEM; n = 6 mice in 2 experiments, each assayed individually). (B) Representative flow plots of cDCs (CD11c⁺ B220⁻) and pDCs (CD11c⁺ B220⁺) in BM. The bar graphs show (left) total cDCs and pDCs per femur, and (right) total cDCs and pDCs in spleen (mean ± SEM; m = 3 mice per experiment, each assayed individually; 2 experiments). (C) Total DCs per femur in mice after treatment with indomethacin with or without dmPGE₂ (mean ± SEM; n = 4 mice per group, each assayed individually). (D) Total DCs per femur in mice after treatment with Flt3L with or without dmPGE₂ (mean ± SEM; n = 4 mice per group, each assayed individually). (E) Representative dot plots of CDP frequency and average total CDPs per femur in the BM after 6 days of indomethacin treatment (mean ± SEM; n = 3 mice/group in each of 2 experiments). (F) Representative dot plots of pre-cDC frequency and total pre-cDCs per femur (mean ± SEM; n = 3 mice/group in each of 2 experiments). V indicates vehicle; Indo, indomethacin. *P < .05.