PAL-E antigen colocalizes with PV-1 and NRP-1. The 2-color immunofluorescent stainings of the indicated human tissues using anti–PV-1, anti–NRP-1, and PAL-E antibodies were performed as described in “Immunostainings” and, in more detail, in supplemental Methods. White arrows indicate the structures, which are positively stained by anti–NRP-1 antibodies but negative for both anti–PV-1 and PAL-E. Original magnifications ×200. Scale bars represent 100 μm.