Colocalization studies demonstrate association of TIMP2, VEGF, and P-selectin with VAMP-7–expressing α-granules. Double fluorescence confocal microscopy of granules in spread platelets stained using antibodies to (A) anti-TIMP2, (B) anti-VEGF, or (C) P-selectin and counterstaining with anti–VAMP-7 antibody demonstrates peripheral colocalization of VAMP-7 with these α-granule markers. Scale bars, 5 μm.