Bach1-deficient mice exhibit decreased severity in the autoimmune disease model EAE. WT-type or Bach1^−/− mice were immunized subcutaneously with MOG_35-55 emulsified in CFA and injected with PT. (A) Bach1^−/− mice had decreased severity of paralysis in the EAE model. After induction of EAE, clinical disease scores were measured. One of 3 independent experiments is shown; each experiment contained ≥ 7 animals per genotype. Two-way ANOVA was used for P value. (B) Bach1^−/− mice were protected from severe paralysis after EAE induction. The graph shows the percentage of animals that reach the corresponding clinical score. The data were generated with > 40 WT animals and Bach1^−/− animals. (C) Bach1^−/− mice were less sick after EAE induction. The weights of the mice were taken at the indicated times after EAE induction and expressed as fraction weight loss. Two-way ANOVA, P < .001 (WT N = 28; Bach1^−/− N = 29). (D) Bach1^−/− animals produced lower amount of IgG antibodies against MOG_35-55. Serum from mice (N = 10) before and 2 weeks after EAE induction were subjected to ELISA analysis to quantify the relative amount of MOG_35-55–specific antibody produced, expressed as OD450 nm in the plot. All graphs are represented with SEM.