Bach1^−/− EAE mice display less inflammation in the CNS than WT animals. Spinal cords of mice were harvested 1 month after EAE induction, and immune cell infiltrates were quantified by FACS. The left graph represents the FACS plots after forward scatter and side scatter gating, and the right plot shows the compiled data of the relative percentage of corresponding immune cells infiltrated into the spinal cord of the EAE animals. (A) Lower APCs (CD45⁺ CD11b⁺ MHC II⁺) in Bach1^−/− mice induced with EAE. Displayed FACS plots are CD45⁺ gated. (B) DCs were distinguished as CD45⁺ CD11c⁺ GR-1⁻ cells. Displayed FACS plots are GR-1⁻ gated. (C) Microglia population was marked as CD45^int CD11b⁺ population. (D) Infiltration of pan T cells in the spinal cord were identified as CD45⁺ CD3ϵ⁺ cells. (E) Th infiltrates were distinguished as CD3e⁺CD4⁺ cells (cell population in pink box). (F) B cells in the spinal cord were identified as CD45⁺ CD19⁺ cells (cell population in pink box). Bolded letters of the surface markers denote that the plot shown are after gating to that particular marker. Each group contains ≥ 15 animals. Unpaired 2-tail Student t test performed for all the panels to obtain P value. The horizontal bars on each graph represents the average percentage.