BM cells were harvested from adult mice and subjected to flow cytometric analyses. (A) Development of macrophages and DCs from HSCs. HSCs give rise to CMP-Flk2⁺. Macrophage-DC-restricted progenitors (MDPs) can generate both macrophages and DCs, whereas common DC progenitors (CDPs) are restricted to formation of DCs. The panels correspond to the following cells: (B) monocytes, (C) pre-cDCs, (D) CDPs, (E) MDPs, (F) CMP-Flk2⁺ cells, (G) HSPCs, and (H) multipotent progenitor (MPP) or short-term hematopoietic stem cells (ST-HSCs). Each plot represents ≥ 3 mice. The graphs are plotted with SEM.