Western blots and identification of TFPI isoforms by deglycosylation for SK–Hep-1 endothelial cells. (A) Recombinant TFPIα, PIPLC-released rTFPIβ, and PIPLC-released SK–Hep-1 TFPI were affinity purified and then treated without (−) or with (+) degylcosylation enzymes and probed using anti-TFPI polyclonal antibodies. (B) PIPLC-released SK–Hep-1 TFPI probed with FLAER and anti-FLAER.