Release of TFPI isoforms from EAhy926 cells by various treatments. EAhy926 cells were treated as indicated with no additions, PIPLC, and PIPLC plus heparin, and releasates were analyzed using immunoassays. Cells treated with no addition and PIPLC plus heparin were collected by centrifugation, washed once, and lysed by 2 freeze-thaw cycles. Lysates were clarified by centrifugation and supernatants analyzed for TFPI isoforms by immunoassays.