Western blots of placental microsomal TFPI. (A) Recombinant TFPIα, PIPLC-released rTFPIβ, and PIPLC-released TFPI from placental microsomes were affinity purified and then treated without (−) or with (+) degylcosylation enzymes and probed using anti-TFPI polyclonal antibodies. (B) Placental microsomal TFPI treated without (−) or with (+) degylcosylation enzymes and probed with FLAER and anti-FLAER.