PCI-32765 inhibits anti-IgM and NLC-mediated prosurvival signals in CLL cells. (A) Contour plots of a representative CLL sample, depicting CLL cell viabilities after 24, 48, and 72 hours of incubation with anti-IgM in the presence or absence of PCI-32765, as indicated above the plots. The gates in each of the plots highlight the viable cell populations. (B-C) Bar diagrams representing the mean relative CLL cell viabilities after 24, 48, and 72 hours of anti-IgM stimulation in the presence or absence of PCI-32765 (B, n = 11) or in NLC cocultures (C, n = 15). Viabilities were normalized to the relative viability of control samples with medium (100%) to account for differences in spontaneous apoptosis in samples from different patients. Displayed are means ± SEM. *P ≤ .05.