Comparison of Ca2+ flux, proliferation, and telomerase activity in individual cases. B-CLL cells from 16 U-CLL and 15 M-CLL cases were analyzed for responsiveness to HB57-dex and to goat anti-μ F(ab′)2. The left side of every panel depicts scattergrams representing fold change compared with unstimulated cells: Ca2+ flux, at 5 minutes after stimulation, and proliferation and telomerase activity after 3 days in culture. Each line connecting 3 points represents a single patient. The right half of every panel depicts box plots of the same data (on the left), indicating mean values (horizontal line inside box) and whiskers representing lowest and highest value for each parameter. The trend of responses is different for the U-CLL (A) and M-CLL (B) cases. B cells from unmutated cases show Ca2+ flux and proliferative response similar to those in the mutated cases, but their telomerase activity is significantly higher than in the mutated cases.
