Killing of C albicans by control and NET-forming neutrophils. (A) C albicans was added to control neutrophils (white bars) and neutrophils forming NETs after incubation for 120 minutes with 20 ng/mL PMA (hatched bars). CD (10 μg/mL) and DNase I (100 U/mL) were added 20 minutes before addition of bacteria. (B) Same as panel A, with killing calculated after DNase I (100 U/mL) was added at the end of incubation with the yeasts to the wells not already containing it. Error bars represent SEM (n = 3).