Gata2 target genes are down-regulated after siRNA knockdown of Gata2. (A) Gata2 mRNA and protein levels are substantially reduced in primary embryonic LECs after transfection with Gata2 siRNA, compared with transfection with control siRNA. (B) Quantification of Kdr, Pecam1, Foxc2, Nfatc1, Angpt, and Itga9 mRNA expression levels by real-time RT-PCR in primary LECs transfected with control and Gata2 siRNA. (C) Prox1 mRNA and protein levels are substantially reduced in primary embryonic LECs after transfection with Gata2 siRNA, compared with transfection with control siRNA that has no established targets. Error bars represent mean ± SEM (n = 3), and data are representative of 3 independent cell isolations from 20 to 30 embryos. *P < .05 (Student paired t test). Quantification of protein levels was calculated after normalization with respect to β-actin and expressed as percentage change from controls. Each lane contains equal amounts of protein pooled from 3 separate transfections.