A model of Gata2 regulated transcription in LECs. Our data demonstrate that GATA2 regulates the expression of key transcription factors important for valve development. GATA2 lies upstream of Prox1, Foxc2, and Nfatc1. FoxC2 and NFATc1 have been demonstrated to interact together to coordinately regulate transcription in LECs. NFATc1 and Gata2 have been found to heterodimerize in myocytes, suggesting that these transcription factors might interact together in LECs. Gata2, Prox1, FoxC2, and NFATc1 have been demonstrated to bind the promoter and/or regulate the transcription of genes, including Pecam1, Kdr, Cdh5, Angpt2, and Itga9.