Syntaxin-11 is recognized by the syntaxin-2 polyclonal Ab. (A) Increasing amounts (2.5, 10, and 20 ng) of recombinant His₆-tagged cytosolic domain of human syntaxin-2 (rStx2, lanes 1-3), human syntaxin-4 (rStx4, lanes 4-6), human syntaxin-7 (rStx7, lanes 7-9), and GST-tagged human syntaxin-11 (rStx11, lanes 10-12) were separated by SDS-PAGE and probed by Western blotting with the indicated Abs. (B) Human platelet extract was incubated with syntaxin-2–conjugated Sepharose beads. The specific bound proteins were eluted and subjected to Western blotting using anti–syntaxin-11 Ab. No Ab (−IgG) and nonspecific IgG (+IgG) controls were included.