The presence of the JAK2^V617F mutation induces an increase in CDC25A protein translation. (A) JAK2^V617F and JAK2^WT-expressing FDC-P1 EPOR cells were incubated with 10 μg/mL of cycloheximide (CHX), an inhibitor of protein translation, harvested at indicated times, and subjected to Western blot analysis of CDC25A (left). Quantification of CDC25A protein levels was performed by densitometric analysis normalized to β-actin level (right). The results are expressed as mean ± SD for 3 independent experiments. (B) JAK2^V617F and JAK2^WT-expressing FDC-P1–EPOR cells were incubated for 1 hour with 10 μg/mL of CHX, then washed (−CHX), and harvested at the indicated times. Cell lysates were prepared and subjected to Western blot to analyze the reappearance of CDC25A (left). CDC25A levels were determined by densitometric analysis normalized to β-actin level (right). The results are the means ± SD for 3 independent experiments. Vertical lines have been inserted to indicate a repositioned gel lane.