Action of 10nM LBH589 on Mcl-1, Bcl-2, mitochondrial membrane depolarization and apotosis in CLL cells. Change in Mcl-1 (A), Bcl-2 (B), and mitochondrial membrane depolarization as measured by loss of the mitochondrial dye TMRM (C) and increase in annexin V/propidium iodide positivity after exposure to 10nM LBH589 for 48 hours (D). (E) Influence of pre-exposure to z-vad-fmk on the action of 10nM LBH589 for 48 hours on Mcl-1, appearance of cleaved caspase-3, and apoptosis in primary CLL cells (n = 4).